From cleanroom to customer: a 25 year journey in biopharmaceuticals My name is Sharief Sahebdin. In March 2025 I joined ROMYNOX to support our single-use customers and share my extensive knowledge of this technology. Over the years I have had the privilege of working closely with a wide range of clients, helping them tackle challenges in demanding processes, especially in the area of safe and efficient fluid transfer. Earlier in my career I stood inside a Grade A cleanroom myself, fully suited in sterile garments, gaining firsthand experience of the strict requirements and precision needed in biopharmaceutical production.
Every movement matters. How you position your arms, how you turn without creating turbulence, how you connect a hose without risking contamination. You cannot just touch or open anything, so you have to stay calm and rely on your knowledge, the process, and your team. These experiences taught me that true expertise comes from understanding why you do something. Why you never cross a sterile boundary, why every deviation must be reported immediately, and why sometimes an entire batch has to be rejected to avoid risks. Everything we do has a direct impact on the patient. Our precision is their safety. That is why I confidently work with technologies like Quattroflow, which support reliability and accuracy in every process.
What makes Quattroflow unique?
Quattroflow is a specialized type of four chamber (quaternary) diaphragm pump, developed specifically for the biopharmaceutical industry. Instead of using rotating impellers, it operates with four flexible diaphragms that move in sequence like a gentle heartbeat. This creates a continuous, pulse free flow, unlike the jolting and surging motion you see with traditional pumps. Because there is virtually no turbulence or shear stress, sensitive fluids are transferred with minimal impact, preserving their structure. Imagine having to pump a delicate cell culture with a standard industrial pump. The result? It would be like forcing a raw egg through a fire hose. This is exactly where Quattroflow makes the difference. The movement of the diaphragms is so smooth and controlled that cells, proteins, or virus particles remain intact.
Another major advantage of Quattroflow is the option for single use pump chambers on many models. You can replace the entire wetted side of the pump within minutes. This eliminates time consuming cleaning cycles and significantly reduces the risk of cross contamination between batches. Since there are no rotating parts or mechanical seals in the product path, there are virtually no areas where microorganisms or residue can hide. Quattroflow pumps can even run dry without causing damage.
Quattroflow in Practice: From Chromatography to Final Filling
While I have not been directly involved in every process, I regularly hear from colleagues and customers about how Quattroflow stands out in critical applications. Here are some common scenarios where this pump technology clearly proves its value:
Chromatography: An unstable flow can disrupt binding in expensive columns and reduce overall yield. With Quattroflow’s stable, pulse free flow, the resin remains undisturbed, allowing the process to achieve maximum efficiency.
Tangential Flow Filtration (TFF): In continuous recirculation, consistent pressure is crucial. Quattroflow prevents aggregation and degradation of sensitive proteins, which helps maintain both yield and quality, even during extended runs.
These examples show how Quattroflow contributes to control, consistency, and product integrity across various bioprocessing steps. It is exactly this level of reliability that makes it a technology I confidently recommend.
Expertise and Partnership
At ROMYNOX it is not just about advanced technology like Quattroflow but about the expertise and guidance that come with it. With over 25 years of experience I help biopharma companies solve complex challenges, such as reducing shear stress or optimizing single use processes. Our goal is not to be a supplier but a partner who stands beside you at every step.
The impact of Quattroflow pumps is clear. They deliver reliable performance, gentle flow and optimal protection of your product. But the true value lies in how you apply the technology. That is where we make the difference through knowledge, dedication and hands-on experience.
Ready to improve your bioprocess with Quattroflow? Feel free to reach out to me or the ROMYNOX team. Want to dive deeper into the technology? A whitepaper is available full of insights and practical tips.
